Evaluation of the Antibacterial Effect of Mixed Lidocagle and Propolis

Su Hyeon Heo1   Sung Suk Bae1,*   

1Department of Dental Hygiene, Hanseo University, Seosan, 31962, Korea

Abstract

This study attempted to verify the antibacterial effect of a lidocagle solution mixed with propolis, at different concentrations, against S. mutans, S. salivarius, and S. mitis. The disk diffusion method, bacterial activity test, and colony formation evaluation assay were performed to evaluate the antibacterial activity of the solutions. Collected data were analyzed by one-way ANOVA and Tukey's post hoc analyses in SPSS 21.0 ver. (SPSS Inc., Chicago, IL, USA); statistical significance was set at 0.05. In the disc diffusion assay, the diameter of the inhibition zone decreased in media containing Lidocagle and propolis at a high concentration (30 vol%). In contrast, in the bacterial activity evaluation test, bacterial activity increased in media containing propolis an high Lidocagle solution concentrations (except for that at 10% vol). In the colony formation evaluation test, the highest number of colonies was formed in media with propolis and a low concentration of the Lidocagle solution (p<0.05). The results suggest that the antibacterial effect depends on the Lidocagle:propoli ratio in the medium. Results also showed that 10%, 20%, and 30% Lidocagle solution volume mixes had partial antibacterial effect against S. mutans, S. salivarius, and S. mitis.

Figures & Tables

Fig. 1. Growth inhibition zones for S. mutans, S. salivarius, and S. mitis, evaluated by disk diffusion method.